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Popular sequences of Short DNA oligonucleotides are frequently used as primers for sequencing cloned genes (Sanger sequencing). Choose between our typically utilized sequencing primers to initiate your sequence verification projects.
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In contrast to random primers, oligo (dT) primers are certainly not suited to degraded samples but are fantastic for constructing cDNA libraries and encouraged to be used with reverse transcriptases (RTs) as Superscript IV RT.
Swimming pools of pre-developed primers may also help make complete expression profiles, Specifically exactly where assay sensitivity is critically critical.
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Contrary to oligo(dT) primers, these primers usually are not template certain and they are well suited for DNA synthesis making use of Klenow fragments with DNA templates or for cDNA synthesis employing reverse transcriptase with mRNA templates.
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Swimming pools of pre-made primers might help deliver complete expression profiles, Specifically where assay sensitivity is critically essential.
Observe that if you purchase Buy ammunition online “NATO” or “military” brass (especially for five.56)…you’re planning to really need to perform some more situation prep considering that they use crimped primers that don’t conveniently assist you to re-prime them with no creating the primer pocket greater.